Genetic variations in the Orf7a protein of SARS-CoV-2 and its possible role in vaccine development

Introduction: SARS-CoV-2 (Severe Acute Respiratory Syndrome) is the causative agent of novel coronavirus disease (COVID-19) that has been creating an unprecedented situation globally. The recurrent mutations in genomes impact on vaccine designing strategies. Orf7a a 121-amino acid-long type I transmembrane accessory protein encoded by genome SARSCoV- 2 and plays crucial role virus–host interaction. present study aimed to analyze variations occurring due multiple its immunological developing promising therapeutic target curb infections. Methods: 16,161 sequences reported from onset this until 13 June 2021 five continents were compared identify genetic protein. Results: A total 470 point detected submitted. Subsequently, nature (deleterious or neutral) was determined. Furthermore, physicochemical properties, antigenicity, allergenicity, toxicity, stability estimated demonstrate Additionally, we identified three B-cell immune epitopes, their MHC cluster analysis also performed. Conclusion: provide deep understanding interactions. Findings our revealed predicted epitopes could be candidates for against COVID-19